Journal: The FASEB Journal

Article Title: Endothelial Exostosin‐1 Is Needed for Glycocalyx Integrity and Inflammatory Resolution in Acute Lung Injury

doi: 10.1096/fj.202601250R

Figure Lengend Snippet: Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining for F4/80 + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.

Article Snippet: Sections were incubated with primary antibodies against CD11b (Abcam, ab16667; 1:200), F4/80 (Bioss, bs‐10701R; 1:200), or heparan sulfate (10E4 clone; Amsbio, #370255; 1:100) overnight at 4°C.

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Cardiovascular Medicine

Article Title: Yiqihuoxue formula improves atherosclerosis by regulating macrophage polarization associated with enhanced STAT6/PPARγ pathway

doi: 10.3389/fcvm.2026.1896890

Figure Lengend Snippet: YQHXF promoted M2 macrophage polarization in mice. ( A and B) Representative immunofluorescence images of macrophage distribution. (C) Immunofluorescence co-staining for detection the number of F4/80 + CD86 + positive cells (M1 macrophages). (D) Immunofluorescence co-staining for detection the number of F4/80 + CD206 + positive cells (M2 macrophages). Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, *** p < 0.001, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. YQHXF, Yiqihuoxue formula.

Article Snippet: Antibodies information: anti-CD86, ER1906-01, 1:200, HUABIO; anti-CD206, ab64693, 1:200, Abcam; F4/80, 28463-1-AP, 1:400, Proteintech; anti-p-STAT6, HA722776, 1:200, HUABIO; anti-PPARγ, 16643-1-ap, 1:200, Proteintech; FITC labeled goat anti-rabbit, GB22303, 1:100, Servicebio; HRP labeled goat anti-rabbit, GB23303, 1:100, Servicebio.

Techniques: Immunofluorescence, Staining, Control

Journal: Frontiers in Cardiovascular Medicine

Article Title: Yiqihuoxue formula improves atherosclerosis by regulating macrophage polarization associated with enhanced STAT6/PPARγ pathway

doi: 10.3389/fcvm.2026.1896890

Figure Lengend Snippet: YQHXF promoted M2 macrophage polarization in HFD-treated mice is associated with STAT6/PPARγ signaling pathway. (A) Representative picture of STAT6, p-STAT6, and PPARγ proteins expression detected by Western blot; (B–D) Quantification of STAT6 (B) , p-STAT6 (C) , and PPARγ (D) proteins relative expression. β-actin was used to normalize the data. (E,F) Representative immunofluorescence images of F4/80 + p-STAT6 + (E) and F4/80 + PPARγ + (F) cells distribution. ( G,H) Quantitative analysis of F4/80 + p-STAT6 + (G) and F4/80 + PPARγ + (H) positive cells. Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, ** p < 0.05, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. HFD: high-fat diet; YQHXF: Yiqihuoxue formula.

Article Snippet: Antibodies information: anti-CD86, ER1906-01, 1:200, HUABIO; anti-CD206, ab64693, 1:200, Abcam; F4/80, 28463-1-AP, 1:400, Proteintech; anti-p-STAT6, HA722776, 1:200, HUABIO; anti-PPARγ, 16643-1-ap, 1:200, Proteintech; FITC labeled goat anti-rabbit, GB22303, 1:100, Servicebio; HRP labeled goat anti-rabbit, GB23303, 1:100, Servicebio.

Techniques: Expressing, Western Blot, Immunofluorescence, Analysis, Control

Journal: Bioactive Materials

Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

doi: 10.1016/j.bioactmat.2026.02.053

Figure Lengend Snippet: Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.

Article Snippet: F4/80 antibody was purchased from Cell Signaling Technology (Danvers MA, Cat #: 70076).

Techniques: Staining, Immunohistochemistry, Expressing